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tsaD [2020-10-31 16:19:10]

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein

Locus

BSU_05940

Isoelectric point

5.02

Molecular weight

36.69 kDa

Protein length

346 aa Sequence Blast

Gene length

1041 bp Sequence Blast

Function

tRNA modification

Product

tRNA modification enzyme

Essential

yes

E.C.

2.3.1.234

Synonyms

ydiE,gcp

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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Categories containing this gene/protein

Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

Groups of genes → Essential genes

Groups of genes → Universally conserved proteins

Gene

Coordinates

643,258 644,298

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed

adenosine³⁷ in tRNA + L-threonylcarbamoyladenylate --> AMP + H⁺ + N⁶-L-threonylcarbamoyladenosine³⁷ in tRNA (according to UniProt)

Protein family

KAE1 / TsaD family (with TsaB, according to UniProt)

Modification

in vitro phosphorylated by TsaE PubMed

Structure

3ZET (from Salmonella typhimurium, 46% identity) PubMed

Localization

cytoplasm (according to UniProt)

Additional information

universally conserved protein

Expression and Regulation